In general, our department focuses on key mechanisms driving cancer metastasis, especially micro RNAs and regulators of tumor-associated proteolysis, and their translational research potential. In this context, our interests generally include the discovery of molecular determinants defining metastasis, molecular determinants of response to novel therapeutics, molecular determinants assisting in metastasis prevention, and molecular staging of cancer.
In the past, we discovered major regulatory mechanisms of one of the most frequently overexpressed receptor molecules within tumor-associated proteolysis, the urokinase receptor (u-PAR), and suggested molecular staging models which can achieve a higher precision in the individual risk classification of patients with gastrointestinal cancers and of minimal residual disease. The group identified the EGFR-targeted therapeutic Cetuximab as an inhibitor of metastasis via blocking u-PAR expression, suggesting u-PAR as a novel biomarker for Cetuximab sensitivity. Also, the group for the first time implicated the anti-malaria agent Artesunate as a metastatis inhibitor, acting via the u-PAR ligand urokinase (u-PA) and specific matrix-metalloproteinases (MMPs). Furthermore, our group pioneered the novel function of tumor suppressor Pdcd4 as a metastasis suppressor by inhibiting u-PAR gene expression, and as an independent prognostic marker in colorectal cancer, and showed that Pdcd4 is essentially downregulated in cancer cells by microRNA-21.
For the recent years, our department puts special emphasis on the discovery of microRNAs (miRs) and their function as key regulators of cancer metastasis. We described microRNA-21 as a key inhibitor of Pdcd4 in colorectal cancer, post-transcriptionally downregulating Pdcd4 expression and stimulating three different steps of the metastastic cascade, invasion, intravasation, and the establishment of metastatic lesions. We also implicated further microRNAs as novel regulators of metastasis of different carcinoma entities, e.g. miR-200c, miR-34a, or the miR-30-family, amongst others. Recently, together with national and international collaborators, we extended out interest to decipher miR-driven molecular networks driving progression and metastasis and suggested a metastatically relevant network of miR-21, miR-34a, Pdcd4, Src, and PTEN. In a recent systematic profiling approach, we discovered and validated several novel miRs to be significantly deregulated in resected colorectal patient metastasis tissue as compared to the matched primary tumors and corresponding normal background tissues. From these, we functionally concluded a first novel molecular network of particular miRs which act, at least in part, via 5 novel, in part common targets, which all culminate in EMT-regulation, orchestrating invasion, in vivo intravasation and metastasis in vivo. To contribute to identifying metastatically relevant individual cells among heterogeneous tumor cell populations, just recently our group presented the first single-cell, single molecule superresolution microscopy of human microRNAs in metastatic cancer cells in a highly interdisciplinary approach with optical biophysics. Ongoing activities in our department include the search for the molecular, genomic and epigenetic causes for miR-deregulation during the metastatic process, and the definition of factors defining site-specific metastasis based on our current data.
Prof. Dr. med. Heike Allgayer, MD, PhD, Director of the Department which is part of the Mannheim Medical Faculty of University of Heidelberg and located at the CBTM, received her MD and Dr. med. at Ludwig-Maximilians University Munich in 1995/1996, and her PhD (in Molecular Biology) at the University of Houston-MD Anderson Cancer Center in 1999. As a board-certified surgeon and molecular biologist, she sees her mission in building the bridge between basic research and metastasis prevention or treatment in cancer patients, as dedicated translational researchers. Amongst other activities and appointments, Heike Allgayer is active member of, for example, the AACR (currently serving as a member of the AACR Surgical Oncology Task Force), ASCO, the Metastasis Research Society (MRS), and EACR (serving as an Council Member and Ambassador), chair of the Fellows of the Ingrid zu Solms Foundation (IzS), and Associate Editor of the International Journal of Cancer (IJC).
Prof. Dr. med. Heike Allgayer, MD, PhD
Department of Experimental Surgery - Cancer Metastasis
Medical Faculty Mannheim
University of Heidelberg
Center for Biomedicine and Medical Technology Mannheim (CBTM)
Tel.: +49 (0)621/383-6876