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Echocardiography

Echocardiography is a robust and reproducible non-invasive approach of assessing cardiovascular function in vivo. Our research group within the ECAS builds on more than 10 years of hands-on experience in murine echocardiography. In our research projects, we use cardiac ultrasound imaging in all stages of cardiac disease as state-of-the-art functional diagnosis tool. Assessed parameters start with systolic function (e.g. left-ventricular ejection fraction) and anatomy (e.g. ventricular dimensions and wall thickness), and lead via diastolic function, speckle-tracking echocardiography and right-ventricular imaging to infant, neonatal and even embryonal stages. One of our research aims is to develop existing echocardiography approaches further and establish new analytical standards in constant exchange with experts from Fujifilm VisualSonics and Purdue University.

Depending on your manuscript or project idea, we will record ultrasound images, assist or conduct the data acquisition and, if requested, give advice in design and structure of experimental groups and cohorts.

Important: In terms of the animal protection law, echocardiography needs to be announced to the regional council and the operating personnel needs to be listed (i.e., us). Depending on the study volume, an extension or new animal proposal will be necessary; however, we will support you in writing.

Services

  • Cardiovascular image acquisition
  • Specific image analysis
  • Guidance in study design for echocardiographic measurements depending on the project hypothesis

Equipment

VisualSonics Vevo 3100

Fujifilm VisualSonics Vevo 3100 System
frequency up to 70 MHz, resolution down to 30 µm

Vevo Imaging Station
including Anesthesia System, Mouse Handling Table with ECG measurement, THM unit ready for third party connection (e.g. DSI, Millar – not provided) and body temperature control

MX400, MX550D and MX700 transducer
covering small rats and large mice, small mice or infant, neonatal and embryonal mice, respectively

Vevo license package
including 1D, 2D Analysis, Doppler imaging, LV tracing and VevoStrain Analysis Suite

Left-ventricular 2-D image (B-mode) in parasternal long axis view
Left-ventricular 2-D image (B-mode) in short axis view
1-D analysis of the left ventricle over time (M-mode) in parasternal long axis view
Pulsed-wave Doppler imaging of the mitral walve flow in apical four chamber view
Post-processing of a short axis image with temporal and spacial differentiation of circumferential strain
3-D graph of circumferential strain rate (strain over time) after post-processing of a short axis image

Publications

Wohlfeil SA, Häfele V, Dietsch B, Schledzewski K, Winkler M, Zierow J, Leibing T, Malek Mohammadi M, Heineke J, Sticht C, Olsavszky V, Koch PS, Géraud C and Goerdt S. Hepatic endothelial Notch activation protects against liver metastasis by regulating endothelial-tumor cell adhesion independent of angiocrine signaling. Cancer Res. 2018; epub ahead of print

Malek Mohammadi M, Kattih B, Grund A, Froese N, Korf-Klingebiel M, Gigina A, Schrameck U, Rudat C, Liang Q, Kispert A, Wollert KC, Bauersachs J and Heineke J. The transcription factor GATA4 promotes myocardial regeneration in neonatal mice. EMBO Mol Med. 2017;9:265-279.

Appari M, Breitbart A, Brandes F, Szaroszyk M, Froese N, Korf-Klingebiel M, Mohammadi MM, Grund A, Scharf GM, Wang H, Zwadlo C, Fraccarollo D, Schrameck U, Nemer M, Wong GW, Katus HA, Wollert KC, Müller OJ, Bauersachs J and Heineke J. C1q-TNF-Related Protein-9 Promotes Cardiac Hypertrophy and Failure. Circ Res. 2017;120(1):66-77.

Zwadlo C, Schmidtmann E, Szaroszyk M, Kattih B, Froese N, Hinz H, Schmitto JD, Widder J, Batkai S, Bähre H, Kaever V, Thum T, Bauersachs J and Heineke J. Antiandrogenic therapy with finasteride attenuates cardiac hypertrophy and left ventricular dysfunction. Circulation. 2015;131(12):1071-81.

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