The SP5 MP is an inverse laser scanning microscope with multiple laser lines for confocal application, an external epi-fluorescence lamp as well as a pulsed IR-laser for multi-photon applications. The visible lasers include an Ar-laser (blue excitation), DPSS laser (light green excitation), HeNe laser (orange and red excitation). The stimulation wavelength of the IR-laser is freely selectable in the range of 690-1040 nm.

In addition, this microscope features a Super Z-Galvo scanning stage which enables a fast acquisition of xz-scans and a resonance scanner, which enables the acquisition of high frame xy rates. The Acusto-optical beam splitter (AOBS) allows a filter-free and sharp distinction of various fluorescence channels. The beam splitter is equipped with four internal fluorescence- and one transmitted-light- detectors for confocal detection as well as two external (Chroma-) filter-based detectors for multi-photon-detection.

This set-up is fully optimized for living cell microscopy. The multi-photon excitation operates in a tissue conserving way and with a high penetration into living tissue or thick samples. It is equipped with a tempered Ludin-incubation chamber with gas mixer, a sterilizable mini-incubation tower for the Z-Galvo stage as well as a super fusion device.

Features

• Research microscope DMI 6000CS Bino, equipped with contrast method Hellfeld, polarization and interference contrast
• External fluorescence lamp EL6000
• Filter system for Epifluorescence
  

  • Blue-application: A (BP 340-380 nm, LP 425 nm)
  • Green-application: I3 (BP 450-490 nm, LP 515 nm)
  • Red-application: N2.1 (BP 515-560 nm, 590 nm), TX2 (BP560/40 nm, LP 645/75 nm)

• Laser lines
  

  • Ar-Laser (458 nm/5 mW; 476 nm/5 mW; 488 nm/20 mW; 496 nm/5 mW; 514 nm/20 mW)
  • DPSS (561 nm/20 mW)
  • HeNe (594 nm/2,5 mW, 633 nm/10 mW)

• Infrared Ti: Saphire Laser MaiTai Deep Sea (variable 690-1040 nm/2,5 W)
• AOBS (0,6-2 nm Selectivity)
• Scanning stage, Super-Z Galvo Stage
• Variable scanner: Image scan: up to 25 images; line scan: up to 2800 images
• Resonance scanner: Image scan: up to 250 images; line scan: up to 16000 images
• 1 TLD-detector for measuring transmitted light images
• 4 internal detector channels for the collection of fluorescence images
• 2 external detector channels for MP
• Lenses: 5x/0.15 NA air; 10x/0.30 NA air; 20x/0.7 NA air; 25x/0.95 NA water; 40x/1.3 NA oil; 40x/1.1 NA water
• Tempered Ludin incubation chamber with gas mixer (Gas concentration selectable 1-15% CO2)
• Sterilizable mini-incubator tower for Z-Galvo stage