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Illumina MiSeq System

The Illumina MiSeq™ System is a desktop next-generation sequencing (NGS) system from Illumina allowing fast and simple targeted or small genome sequencing. The main applications include targeted resequencing, metagenomic analysis and small whole-genome sequencing.

MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 x 300 bp read length. The sequencing technology is based surface-based bridge amplification for clonal amplification and sequencing of each generated cluster using the Illumina "sequencing by synthesis" (SBS) method with reversible terminator chemistry.

Data Generation

Number of reads per run

  • Up to 25 million reads per run (eg, clusters passing filter) 

Throughput per run

  • 25 GB data per 2 × 300 bp run (depending on the kit)

Instrumentation

Illumination

  • 12 light-emitting diodes at 520 nm, 650 nm

Instrument Control Computer

  • Base Unit: Intel Core i7-2710QE 2.10 GHz, 16 GB RAM Memory, 750 GB Hard Drive

Sequencing run time

  • ~ 5.5 hours for a 2 × 25 bp paired-read sequencing run using MiSeq Reagent Kit v2
  • ~ 24 hours for a 2 × 150 bp paired-read sequencing run using MiSeq Reagent Kit v2
  • ~ 39 hours for a 2 × 250 bp paired-read sequencing run  using MiSeq Reagent Kit v2
  • ~ 21 hours for a 2 × 75 bp paired-read sequencing run using MiSeq Reagent Kit v3
  • ~ 56 hours for a 2 × 300 bp paired-read sequencing run using MiSeq Reagent Kit v3
  • ~ 19 hours for a 2 × 150 bp paired-read sequencing run using MiSeq Reagent Kit v2 Micro
  • ~ 17 hours for a 2 × 150 bp paired-read sequencing run using MiSeq Reagent Kit v2 Nano
  • ~ 28 hours for a 2 × 250 bp paired-read sequencing run using MiSeq Reagent Kit v2 Nano

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