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Zebrafish Core Unit

It is the aim of the Zebrafish Core Unit to provide researchers of the Medical Faculty Mannheim with an in vivo platform to study the mechanisms' underlying development and disease and to provide support with target identification and validation. The high fecundity rates, transparent embryos, short generation time, and hardiness make zebrafish optimal model organisms for studying many research areas.

The core facility will allocate and distribute zebrafish embryos of different developmental stages for expression studies, will perform injections of cDNA, mRNAs, transgenic constructs and morpholino antisense oligos for gain-of-function and loss- of-function experiments, and will generate zebrafish mutant lines using the CRISPR/ Cas9 gene editing technology. In addition, the core facility will provide high-throughput screens of chemical libraries.

How do I work with the Zebrafish Core Unit?

  • Collaboration Model
    • Project plans together with core leaders
    • Lab members of core unit will perform experiments
    • Regular meetings/progress reports
    • Collaborative publications
    • Fees: no fees for zebrafish maintanance, but project-specific reagents will be paid by PIs
  • Independence Model
    • Project plans together with core leaders
    • Reseachers will be introduced into the facility and learn how to work with zebrafish by core members
    • Afterwards: reseachers work independently
    • Regular meetings with core leaders
    • Fees: specific reagents, no fees for zebrafish maintanance but support feeding of zebrafish

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