University of Heidelberg
Faculty of Medicine Mannheim
University Hospital Mannheim
News
Dr. Mathias Davids wins the I. I. Rabi Award of the International Society for Magnetic Resonance in Medicine (ISMRM),
read more, article in local newspaper (Mannheimer Morgen)
Keine Gesundheitsgefährdung für Frauen mit implantierten kupferhaltigen Verhütungsmitteln bei der MRT,
read more
CKM @ MEET and CODE,
read more
Job offers now collected in new page on website
Note


If you have questions concerning a specific publication please use this form with subject 'information about publications' and giving the full citation in the message body.

Links
Home > Publications > Abstract >

{A}utomatic {S}egmentation of {U}nstained {L}iving {C}ells in {B}right-{F}ield {M}icroscope {I}mages

M. Tscherepanow, F. Zöllner and F. Kummert

Workshop on Mass-Data Analysis of Images and Signals in Medicine, Biotechnology and Chemistry MDA,, pp.86-95

The automatic subcellular localisation of proteins in living cells is a critical step to determine their function. The evaluation of fluorescence images constitutes a common method of localising these proteins. For this, additional knowledge about the position of the considered cells within an image is required. In an automated system, it is advantageous to locate and segment these cells in bright-field microscope images taken in parallel with the fluorescence micrographs. Unfortunately, currently available cell segmentation methods are only of limited use within the context of protein localisation, since they frequently require microscopy techniques that enable images of higher contrast (e.g. phase contrast microscopy or additional dyes) or can merely be employed with too small magnifications. Therefore, this article introduces a novel approach for the robust automatic segmentation of unstained living cells in bright-field microscope images.

Contact: Prof. Dr. Frank Zöllner last modified: 20.08.2019
to top of page