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Inhalt

Techniques

Single Molecule Level

  • expression (RT-PCR, Western)
  • localisation (Histology)
  • activity (ELISA, single channel patch clamp)

Intact Cell

  • cell-culture
  • mechanotransduction (flow)
  • ion currents (whole cell patch clamp)
  • intracellular ions (calcium, protons)

Intact Organ

  • flow-induced responses
  • vessel contractility
  • membrane potential
  • fluorimetry (calcium, protons)
  • Langendorff heart

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