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Mini Symposium Zebrafish Core Unit

Montag, 15.01.2018, 13:30 - 16:00 Uhr

Ort: CBTM, Tridomus, Building B, Cafeteria, Ludolf-Krehl-Straße 7-11

Agenda

It is the aim of the Zebrafish Core Unit to provide researchers of the Medical Faculty Mannheim with an in vivo platform to study the mechanisms' underlying development and disease and to provide support with target identification and validation.The high fecundity rates, transparent embryos, short generation time, and hardiness make zebrafish optimal model organisms for studying many research areas.

The core facility will allocate and distribute zebrafish embryos of different developmental stages for expression studies, will perform injections of cDNA, mRNAs, transgenic constructs and morpholino antisense oligos for gain-of-function and loss-of-function experiments, and will generate zebrafish mutant lines using the CRISPR/ Cas9 gene editing technology. In addition, the core facility will provide high-throughput screens of chemical libraries.

Schedule

13:30 - 13:40 Welcome Address
CBTM Director Prof. R.-D. Treede

13:40 - 13:55 Jens Kroll and Gergana Dobreva
Zebrafish Core Unit Mannheim - Introduction, Facility,
History, Services

13:55 - 14:00 Discussion

14:00 - 14:15 Jens Kroll
Models for Vascular and Renal Development, Disease
Models for Diabetes Research and Tumor Biology,
Imaging, Mutagenesis (CRISPR/Cas9)

14:15 - 14:25 Discussion

14:25 - 14:40 Gergana Dobreva
Models for Cardiac Development and Stem Cell
Biology, Disease Models for Cardiology, Regenerative
Biology, Imaging, Transgenic lines

14:40 - 15:00 Discussion

15:00 - 16:00 On-Site visit of the Zebrafish Core Unit
Organized by lab members in small groups

In parallel
15:00 - 16:00 Discussions and collaborations
Gergana Dobreva and Jens Kroll

Invitation MiniSymposium ZebraFish

 

 

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