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3D cell culture models from head and neck tumor for personalized medicine

Graduate: Emma Sohn

Our work focuses on patient – derived 3D cell culture models and the evaluation of their potential use in personalized therapy of head and neck cancer. We are particularly interested in their ability to predict response to established therapeutic approaches in high – throughput screenings.

Generation of patient – derived 3D cell culture models from head and neck tumor specimens. Tissue samples are manually dissected into small pieces, which are subjected to histologic analysis, organotypic culture, enzymatic digestion and outgrowth culture. Cells obtained from the latter two are used to generate three – dimensional tumor models. Mononuclear immune cells are added to recapitulate tumor -immune cell interactions in the tumor microenvironment. Anti – cancer drugs are added to the cell culture models and the constructs are subjected to various assays to evaluate the predictive power of the models.
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