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Proteomics

The Proteomics Core Facility (PCF) provides mass spectrometry-based identification, characterization and quantification of proteins and peptides as part of an in depth analysis of highly purified or complex specimens. To a limited extent, the analysis of small molecules, such as metabolites and xenobiotica, is supported as well.

The PCF aids in obtaining competitive results for publication as well as for funding application. Please contact the PCF early in your experimental planning, as PCF experts may provide you with valuable advice to maximize your experimental efficiency and success rate. Furthermore, they are familiar with the current state of proteomics and gladly consult on the state-of-the-art. 

Services

  • Protein identification by mass spectrometry
  • Proteome profiling
  • Large-scale analysis of selected post-translational modifications
  • Intact protein analysis
  • Comparative proteomics (currently limited to scheduled collaborations)

Equipment

Mass Spectrometry

AmaZon Speed (Bruker)
LTQ-Orbitrap XL (Thermo Fisher Scientific) with a chip interface (TriVersa NanoMate; Advion)
Ultraflex I MALDI-TOF MS (Bruker) with Twister unit

Liquid-Based Protein Separation

1200 Infinity HPLC (Agilent)
Nano HPLC (Ultimate, Dionex)

Other

Biosensor (Biacore)
Luminex 200 Multiplex Bead Array (Merck - Millipore)
UV - VIS plate-reader (Victor III, Perkin Elmer)
 

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