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Identification of tissue specific changes within the nociceptive system in diabetic peripheral neuropathy – characterizing pain in zebra fish
Supervisor (Mannheim): Rolf-Detlef Treede
Co-Supervisor (Groningen): Jan-Luuc Hillebrands
Graduate: Jonathan Husk
Project description
In this project we will investigate three major questions characterizing the single zebrafish orthologous of TRPV1/2, a putative evolutionary precursor of the mammalian TRPV1 and TRPV2 and its role in diabetic neuropathy: (i) which are adequate stimuli to activate the TRPV1 channel analogon from zebra fish when heterologously expressed, (ii) how may those stimuli be used in zebra fish behavioral experiments using wild-type and fishes lacking this receptor (both in larvae and adults), and (iii) how are those behavior tests affected in zebra fish models for diabetes type I?
References
- Gau et al J Neurosci 2013;33:5249-60.
- Soares et al 2019 Chem Biol Interact;299:37-43.
- *Sturm et al 2019 Cornea;doi: 10.1097/ICO.0000000000001949 [Epub]
- *Kopf et al Diabetes Res Clin Pract 2018;146:191-201.
- *Vollert et al 2017 Pain;158:1446-1455.
- *Henrich et al 2015 Brain;138:2505-20.
- *Maurer et al 2014 Neurosci Lett;563:101-6.
- *Tsagogiorgas et al 2012 PLoS One;7:e43525.
- *Fischbach et al 2007 J Neurophysiol;98:929-38.
* Own publications
Methods used
Establishing expression systems (HEK293), fluorescent calcium imaging, immunohistochemistry, behavioral experiments in zebrafish and PDX(-/-) KOs (T1DM)
Collaboration Partners
- Guido Krenning, Groningen
- Jacob van den Born, Groningen
- Jens Kroll, Mannheim
- Hans-Peter Hammes, Mannheim
- Benito Yard, Mannheim
- Peter Nawroth, Heidelberg