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MD SP15

Identification of tissue specific changes within the nociceptive system in diabetic peripheral neuropathy – characterizing pain in zebra fish

Supervisor (Mannheim): Rolf-Detlef Treede
Co-Supervisor (Groningen): Jan-Luuc Hillebrands
Graduate: Jonathan Demmer

Project description

In this project we will investigate three major questions characterizing the single zebrafish orthologous of TRPV1/2, a putative evolutionary precursor of the mammalian TRPV1 and TRPV2 and its role in diabetic neuropathy: (i) which are adequate stimuli to activate the TRPV1 channel analogon from zebra fish when heterologously expressed, (ii) how may those stimuli be used in zebra fish behavioral experiments using wild-type and fishes lacking this receptor (both in larvae and adults), and (iii) how are those behavior tests affected in zebra fish models for diabetes type I?

References

  1. Gau et al J Neurosci 2013;33:5249-60.
  2. Soares et al 2019 Chem Biol Interact;299:37-43.
  3. *Sturm et al 2019 Cornea;doi: 10.1097/ICO.0000000000001949 [Epub]
  4. *Kopf et al Diabetes Res Clin Pract 2018;146:191-201.
  5. *Vollert et al 2017 Pain;158:1446-1455.
  6. *Henrich et al 2015 Brain;138:2505-20.
  7. *Maurer et al 2014 Neurosci Lett;563:101-6.
  8. *Tsagogiorgas et al 2012 PLoS One;7:e43525.
  9. *Fischbach et al 2007 J Neurophysiol;98:929-38.

 * Own publications

Methods used

Establishing expression systems (HEK293), fluorescent calcium imaging, immunohistochemistry, behavioral experiments in zebrafish and PDX(-/-) KOs (T1DM)

Collaboration Partners

  • Guido Krenning, Groningen
  • Jacob van den Born, Groningen
  • Jens Kroll, Mannheim
  • Hans-Peter Hammes, Mannheim
  • Benito Yard, Mannheim
  • Peter Nawroth, Heidelberg

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