Confocal microscopes

A Leica TCS SP5 MP is an inverted confocal microscope with a tunable infrared laser for multiphoton imaging, motorised stage and full incubation chamber (deep tissue pentration, maximum confocality).

Microscope characteristics:

• Laser lines: Ar-Laser (568 nm/5 mW; 476 nm/5 mW; 488 nm/20 mW; 496 nm/5 mW; 514 nm/20 mW), DPSS (561 nm/20 mW), HeNe (594 nm/2,5 mW, 633 nm/10 mW)- Infrared Ti: Saphire Laser MaiTai Deep Sea (variable 690-1040 nm/2,5 W)

• Objectives: 5x/0.15 NA air; 10x/0.30 NA air; 20x/0.7 NA air; 25x/0.95 NA water; 40x/1,3 NA oil; 40x/1,1 NA water

• Serial Number: 5100001566, Internal Number: LMI-0919

• Location: Tridomus, C-1-103

A multipurpose instrument for live-cell imaging (environment control modules available) or fixed tissue. It can be used as an inverted confocal microscope, or a combined bright field (phase contrast as well) and fluorescent microscope. It is equipped with 4 laser lines, two sensitive GaAsP-PMT detectors, an Airyscan module (various purposes), and independent components for bright field acquisition (ESID). The microscope is equipped with a motorized XY-stage. Includes wide-field fluorescence component for focusing of standard dyes (DAPI-like, GFP-like, RFP-like).

Main purposes: live cell imaging (time-lapse experiments for bright field, phase contrast and fluorescence), multicolor high-resolution acquisition (up to 0.14 µm), optical sectioning of fixed and living tissue (max. Z-resolution 0.4 µm).

Microscope characteristics:

• Illumination:
  • Lasers (wavelength, nm): 405, 488, 561 and 640
  • White light: ESID (LED)
• Objectives (N.A., immersion medium):
  • 10x (0.45, DRY)
  • 20x (0.8, DRY)
  • 40x (1.30, OIL)
  • 63x (1.40, OIL)
• Emission filters (wavelengths, nm):
  • SP 470 (Em. 410-470).
  • SP 545 (Em. 410-564).
  • SP 620 (Em. 410-617).
  • LBF 640 (Em. 410-617 & 656-750).
  • LP 575 (Em. 576-750).
  • LP 655 (Em. 656-750).
  • 2xVSD (for custom emission detection).
  • Beam splitters for laser lines (405, 488, 561, and 640).
• Detectors:
  • ESID-detector (brightfield).
  • 2 GaAsP-PMTs.
  • Airyscan: a versatile module detector that can be used as an additional GaAsP, as single detector for increased resolution, or signal-to-noise ratio improvement (virtual pinhole).
• Additional features: incubation hood and controlled environment for long-term live cell imaging (humidity, CO₂ and temperature control in hood and stage).
• Serial number: 2633000014, Internal number: FAK-0564
• Location: Tridomus C, C-102

This compact microscope enable the imaging of living cells under physiological conditions (CO₂, temperature and humidity control) and is suitable for fixed tissue in bright field or fluorescent mode (wide field and confocal).

Microscope characteristics:

• Illumination: 4 LED-sources: 365/400/555/625 nm
• 4 Diode Lasers: 405nm, 488nm, 561nm and 638nm
• Objectives (N.A., immersion medium):
  • 1,6x (0.05, DRY)
  • 10x (0.32, DRY)
  • 20x (0.75 CS2, DRY)
  • 63x (1.20 CS2, water)
• Detectors:
  • 4 cameras for wide field
  • 4 HyD detectors for the confocal mode
  • Carrier type:
  • Glass slides
  • Multi chamber slides (e.g. IBIDI)
  • Imaging dishes
  • Multiwell plates
  • Multiholder available (3 slides)
• Additional features: Micro hub is an incubation hood and controlled environment for live cell imaging (humidity, CO₂ and temperature control). Fully automated acquisition via user-friendly interface. On-stream dye and background deconvolution.
• Serial number:  539550,  Internal number: LMI-1228
• Location: House 42, Room: 42-3-3226

The TCS SP5 DS is an upright laser scanning microscope with a motorized XY-stage.

Microscope characteristics:

• Filters: DAPI,GFP;Rhodamine    
• Objectives: EC PLnN 2,5x/0,075; EC PLnN 10x/0,3 DIC I; EC PLnN 20x/0,5 DIC II; EC PLnN 40x/0,75 DIC II; PLn Apo 63x/1,4 Oil DIC II; EC PLn 100x/1,3 Oil DIC II 
• Serial Number: 3512001627, Internal Number: LMI-0838
• Location: Tridomus, C-104

The TCS SP8 is an upright laser scanning microscope with a motorized XY-stage

Microscope characteristics:

• Lasers: 405, 488, 552 and 638nm 
• Objectives: 20x/0,70IMM; 40x/1,25 Oil; 63x/1,32 Oil; 10x/0,3 Water and 25x clearing
• Serial Number: 8100001172, Internal Number: LMI 1000-900-01
• Location: House 42, R3225

The TCS SP8 DLS from Leica Microsystems integrate the Light Sheet Microscopy technology into the confocal microscope.It could be used also as  a normal inverse confocal microscope. For Light Sheet the specimen needs to be placed into the focal plane of the detection objective in between the two mirrors of the cap.

Microscope characteristics:

• Lasers: 488nm, 552nm und 638nm
• Filters: FITC LP (Excitation: BP 470/40)
• Emission: LP 515): RHOD LP (Excitation: BP 540/45, Emission: LP 590)
• Detection objective: HC PL FLUOTAR 5x/0,15 (free working distance: 4,95mm); HC FLUOTAR L 16x/0,6 IMM CORR
• Illumination objectives: 1,6x/0,05 (und 10/20/40 /63 (all ceramic objectives))
• Serial Number: 8110002056, Internal Number: LMI 1043-160-1
• Location: House 42, R3225