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Confocal microscopes

Confocal suitable for live cell imaging

Leica Confocal Microscope (inverted, TCS SP5 MP (Multiphoton))

A Leica TCS SP5 MP is an inverted confocal microscope with a tunable infrared laser for multiphoton imaging, motorised stage and full incubation chamber (deep tissue pentration, maximum confocality).

Microscope characteristics:

  • Laser lines: Ar-Laser (568 nm/5 mW; 476 nm/5 mW; 488 nm/20 mW; 496 nm/5 mW; 514 nm/20 mW), DPSS (561 nm/20 mW), HeNe (594 nm/2,5 mW, 633 nm/10 mW)- Infrared Ti: Saphire Laser MaiTai Deep Sea (variable 690-1040 nm/2,5 W)

  • Objectives: 5x/0.15 NA air; 10x/0.30 NA air; 20x/0.7 NA air; 25x/0.95 NA water; 40x/1,3 NA oil; 40x/1,1 NA water

  • Serial Number: 5100001566, Internal Number: LMI-0919

  • Location: Tridomus, C-1-103

LSM800, Zeiss

A multipurpose instrument for live-cell imaging (environment control modules available) or fixed tissue. It can be used as an inverted confocal microscope, or a combined bright field (phase contrast as well) and fluorescent microscope. It is equipped with 4 laser lines, two sensitive GaAsP-PMT detectors, an Airyscan module (various purposes), and independent components for bright field acquisition (ESID). The microscope is equipped with a motorized XY-stage. Includes wide-field fluorescence component for focusing of standard dyes (DAPI-like, GFP-like, RFP-like).

Main purposes:

  • live cell imaging (time-lapse experiments for bright field, phase contrast and fluorescence), multicolor high-resolution acquisition (up to 0.14 µm), optical sectioning of fixed and living tissue (max. Z-resolution 0.4 µm).

Microscope characteristics:

  • Illumination:
    • Lasers (wavelength, nm): 405, 488, 561 and 640
    • White light: ESID (LED)
  • Objectives (N.A., immersion medium):
    • 10x (0.45, DRY)
    • 20x (0.8, DRY)
    • 40x (1.30, OIL)
    • 63x (1.40, OIL)
  • Emission filters (wavelengths, nm):
    • SP 470 (Em. 410-470).
    • SP 545 (Em. 410-564).
    • SP 620 (Em. 410-617).
    • LBF 640 (Em. 410-617 & 656-750).
    • LP 575 (Em. 576-750).
    • LP 655 (Em. 656-750).
    • 2xVSD (for custom emission detection).
    • Beam splitters for laser lines (405, 488, 561, and 640).
  • Detectors:
    • ESID-detector (brightfield).
    • 2 GaAsP-PMTs.
    • Airyscan: a versatile module detector that can be used as an additional GaAsP, as single detector for increased resolution, or signal-to-noise ratio improvement (virtual pinhole).
  • Additional features: incubation hood and controlled environment for long-term live cell imaging (humidity, CO2 and temperature control in hood and stage).
  • Serial number: 2633000014, Internal number: FAK-0564
  • Location: Tridomus C, C-102

Leica Mica Microhub

This compact microscope enable the imaging of living cells under physiological conditions (CO2, temperature and humidity control) and is suitable for fixed tissue in bright field or fluorescent mode (wide field and confocal).

Microscope characteristics:

  • Illumination: 4 LED-sources: 365/400/555/625 nm
  • 4 Diode Lasers: 405nm, 488nm, 561nm and 638nm
  • Objectives (N.A., immersion medium):
    • 1,6x (0.05, DRY)
    • 10x (0.32, DRY)
    • 20x (0.75 CS2, DRY)
    • 63x (1.20 CS2, water)
  • Detectors:
    • 4 cameras for wide field
    • 4 HyD detectors for the confocal mode
    • Carrier type:
    • Glass slides
    • Multi chamber slides (e.g. IBIDI)
    • Imaging dishes
    • Multiwell plates
    • Multiholder available (3 slides)
  • Additional features: Micro hub is an incubation hood and controlled environment for live cell imaging (humidity, CO2 and temperature control). Fully automated acquisition via user-friendly interface. On-stream dye and background deconvolution.
  • Serial number:  539550,  Internal number: LMI-1228
  • Location: House 42, Room: 42-3-3226

Upright Confocal

Leica Confocal Microscope (upright, TCS SP5 DS)

The TCS SP5 DS is an upright laser scanning microscope with a motorized XY-stage.

Microscope characteristics:

  • Filters: DAPI,GFP;Rhodamine    
  • Objectives: EC PLnN 2,5x/0,075; EC PLnN 10x/0,3 DIC I; EC PLnN 20x/0,5 DIC II; EC PLnN 40x/0,75 DIC II; PLn Apo 63x/1,4 Oil DIC II; EC PLn 100x/1,3 Oil DIC II 
  • Serial Number: 3512001627, Internal Number: LMI-0838
  • Location: Tridomus, C-104

Leica Confocal Microscope TCS SP8 (upright)

The TCS SP8 is an upright laser scanning microscope with a motorized XY-stage

Microscope characteristics:

  • Lasers: 405, 488, 552 and 638nm 
  • Objectives: 20x/0,70IMM; 40x/1,25 Oil; 63x/1,32 Oil; 10x/0,3 Water and 25x clearing
  • Serial Number: 8100001172, Internal Number: LMI 1000-900-01
  • Location: House 42, R3225

Inverted Confocal

LSM800, Zeiss

A multipurpose instrument for live-cell imaging (environment control modules available) or fixed tissue. It can be used as an inverted confocal microscope, or a combined bright field (phase contrast as well) and fluorescent microscope. It is equipped with 4 laser lines, two sensitive GaAsP-PMT detectors, an Airyscan module (various purposes), and independent components for bright field acquisition (ESID). The microscope is equipped with a motorized XY-stage. Includes wide-field fluorescence component for focusing of standard dyes (DAPI-like, GFP-like, RFP-like).

Main purposes:

  • live cell imaging (time-lapse experiments for bright field, phase contrast and fluorescence), multicolor high-resolution acquisition (up to 0.14 µm), optical sectioning of fixed and living tissue (max. Z-resolution 0.4 µm).

Microscope characteristics:

  • Illumination:
    • Lasers (wavelength, nm): 405, 488, 561 and 640
    • White light: ESID (LED)
  • Objectives (N.A., immersion medium):
    • 10x (0.45, DRY)
    • 20x (0.8, DRY)
    • 40x (1.30, OIL)
    • 63x (1.40, OIL)
  • Emission filters (wavelengths, nm):
    • SP 470 (Em. 410-470).
    • SP 545 (Em. 410-564).
    • SP 620 (Em. 410-617).
    • LBF 640 (Em. 410-617 & 656-750).
    • LP 575 (Em. 576-750).
    • LP 655 (Em. 656-750).
    • 2xVSD (for custom emission detection).
    • Beam splitters for laser lines (405, 488, 561, and 640).
  • Detectors:
    • ESID-detector (brightfield).
    • 2 GaAsP-PMTs.
    • Airyscan: a versatile module detector that can be used as an additional GaAsP, as single detector for increased resolution, or signal-to-noise ratio improvement (virtual pinhole).
  • Additional features: incubation hood and controlled environment for long-term live cell imaging (humidity, CO2 and temperature control in hood and stage).
  • Serial number: 2633000014, Internal number: FAK-0564
  • Location: Tridomus C, C-102

Fluoview FV3000RS, Evident (former Olympus)

The FLUOVIEW FV3000RS is a confocal point laser scanning microscopes, enabling a wide range of imaging modalities which include macro-to-micro imaging, multiplexing, spectral unmixing, high resolution volumetric imaging, and fast image acquisition.

Main features (purposes):

  • Standard Galvo scanner for high-resolution three-dimensional imaging
  • Resonant scanner for fast imaging of cellular events
  • Four spectral high-sensitive detectors (HSD, type GaAsP-PMT) for imaging weak fluorescence signals
  • Multi-channel experiments (multiplexing) with assigning up to 4 channels to each detector.
  • Each confocal detector of the FV3000RS is equipped with a highly light efficient spectral dispersion element (VPH, Volume Phase Holographic grating) that allows for adjust the spectral detection window from 1 – 100 nm with 1 nm increments.
  • The Experiment Sequencer (i.e., a hardware sequencing software module) allows to execute different imaging and/or light stimulation tasks for a variety of different applications.

Microscope characteristics:

  • Illumination:
    • Diode lasers (wavelength, nm)
    • Main combiner: 405/488/552/640 nm
    • Sub combiner: 445/514/594 nm
  • Extended Super-Apochromat objectives (N.A., immersion medium, cover glass correction 0.17 mm):
    • UPlanXApo 10x/0.40, ∞/0.17/OFN26.5
    • UPlanXApo 20x/0,80, ∞/0.17/OFN26.5
    • UPlanSApo 40x/1,25 Sil, ∞/0.13-0.19/FN22 (silicone oil)
    • UPlan XApo 60x/1.42 Oil, ∞/0.17/OFN26.5
  • Differential Interference Contrast (DIC) Prisms:
    • IX2-DIC10 DIC Prisma
    • IX2-DIC20 DIC Prisma
    • IX2-DIC40 DIC Prisma
    • IX2-DIC60 DIC Prisma
    • Filter cubes for epifluorescence observation (widefield) by eyepieces:
    • U-FUW FL (UV excitation, e.g., DAPI) 2711900 (340-390 nm ex/410 nm em)
    • U-FBN FL (blue excitation, e.g., GFP, Alexa488) N2712700 (470-495 nm ex/405 nm em)
    • U-FGWA FL (green excitation, e.g., TRITC) l 2713000 (530-550 nm ex/570 nm em)
  • Detectors:
    • 4 spectral high-sensitive GaAsP-PMT detectors (HSD1/HSD2/HSD3/HSD4)
    •  Transmitted light detector (TD, type multi-alkali-PMT)
  • Scanner:
    • Hybrid Scan Unit (HSU) with standard galvo scanner and fast resonant scanner (both FN18). The galvo scanner has a scan-field resolution from 64x64x to 4096x4096 pixel. The resonant scanner has a scan field resolution of 512x512 pixel with up to 30 fps at full scan field and up to 438 fps clipped.
  • Additional features:
    • HGLGPS Light source for epifluorescence (widefield) and 3 filtercubes for observation with eyepieces.
  • Serial number: 9M89759
  • Internal number: LMI-1162
  • Location: Tridomus House B, Room: B-216/217

Leica Light Sheet Microscope TCS SP8 DLS (inverted Microscope DMi9 CS Bino DLS)

The TCS SP8 DLS from Leica Microsystems integrate the Light Sheet Microscopy technology into the confocal microscope.It could be used also as  a normal inverse confocal microscope. For Light Sheet the specimen needs to be placed into the focal plane of the detection objective in between the two mirrors of the cap.

Microscope characteristics:

  • Lasers: 488nm, 552nm und 638nm
  • Filters: FITC LP (Excitation: BP 470/40)
  • Emission: LP 515): RHOD LP (Excitation: BP 540/45, Emission: LP 590)
  • Detection objective: HC PL FLUOTAR 5x/0,15 (free working distance: 4,95mm); HC FLUOTAR L 16x/0,6 IMM CORR
  • Illumination objectives: 1,6x/0,05 (und 10/20/40 /63 (all ceramic objectives))
  • Serial Number: 8110002056, Internal Number: LMI 1043-160-1
  • Location: House 42, R3225
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