Step 1: Service registration

1. Make an appointment with LIMa staff via lima@remove-this.medma.uni-heidelberg.de for an Initial User Meeting

2. Consent to LIMa User Agreements and complete the registration form with signatures

3. Provide the information on the origin of the samples (filling “Formblatt Aufz Core Facility”) when working with genetically modified organism (GMO) Zimmermann (House 42)

Step 2: Safety briefings

1.  Laser safety briefing: held annually, mandatory for users working with confocal microscopy systems or other instruments including lasers

2. General safety briefing: mandatory for all users; responsible persons are Dr. Karin Haack, Ina Schäfer (Tridomus) and Camela Jost (House 42)

3. Facility access control: contact persons are Klaus Lehmann (Tridomus) and Dr. Silvia Zimmermann (House 42)

Step 3: Microscope training

The user will be granted access to the microscope after completing training specific to that model. Please contact LIMa staff to schedule training appointments.

Step 4: Booking microscopes

Microscopes should be booked on Clustermarket: https://app.clustermarket.com/login

Please note that a booking has a maximum duration of four hours between 7am and 7pm; extended sessions are possible outside of this 12h time window (e.g., for time-lapse recordings).

In addition, cases listed below are subject to prior approval by the CF management:

• More than three bookings per week per user
• Early booking (more than 6 weeks in advance)
• Serial consecutive bookings (e.g., by users from the same group)
• Parallel bookings of confocal microscopes

Detailed information and rules about booking microscopes can be found in the User Agreements.